Team:Paris Liliane Bettencourt/Notebook/2011/07/04/

From 2011.igem.org

Team IGEM Paris 2011

Minipreps, digestion, electrophoresis and purification

Axel

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=248.8ng/µL
  • on BBa_K143079 (device with constitutive promoter and GFP), concentration of DNA=500.2ng/µL
  • on BBa_B0015, concentration of DNA=408.1ng/µL
  • on BBa_E1010 concentration of DNA=330.8ng/µL
  • on BBa_K145001, concentration of DNA=572.8ng/µL


digestion

  • BBa_K090403 cut by EcoR1 and XbaI
  • BBa_K143079 cut by EcoR1 and SpeI
  • BBa_B0015 cut by EcoR1 and XbaI
  • BBa_E1010 cut by EcoR1 and SpeI
  • BBa_K145001 cut by EcoR1 and SpeI


Electrophoresis 040711.jpg

gel electrophoresis at 0.6% of Agarose

  • well 1: ladder 1kb
  • well 2: BBa_B0015 cut by EcoR1 and XbaI
  • well 3: BBa_E1010 cut by EcoR1 and SpeI didn't work
  • well 4: BBa_K145001 cut by EcoR1 and SpeI
  • well 5: BBa_K090403 cut by EcoR1 and XbaI
  • well 6: BBa_K143033 cut by EcoR1 and SpeI


gel purification

  • well 1: ladder 1kb
  • from well 2: BBa_B0015 cut by EcoR1 and XbaI, concentration of DNA=10.9ng/µL
  • from well 4: BBa_K145001 cut by EcoR1 and SpeI, concentration of DNA=8.5ng/µL
  • from well 5: BBa_K090403 cut by EcoR1 and XbaI, concentration of DNA=9.2ng/µL
  • from well 6: BBa_K143033 cut by EcoR1 and SpeI, concentration of DNA=5.3ng/µL

Babak

Minipreps

  • on BBa_K090403 (vector), concentration of DNA=174ng/µL
  • on BBa_J18913 (T7 Terminator), concentration of DNA=149ng/µL
  • on BBa_B0015, concentration of DNA=375ng/µL
  • on BBa_E1010 (RFP) concentration of DNA=50g/µL
  • after Et-OH reconcentration BBa_E1010: 80ng/µL

digestion

  • well 2: BBa_E1010 cut by EcoR1 and SpeI
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