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From 2011.igem.org

 We autoclaving 1.5ml eppendorff tubes and then were treat at 800C for 2h in the microwave for nuclease elimination.

 Orlando prepare chloramphenicol (100µg/ml)

 We pass our Biobrick from Agar medium + chloramphenicol to LB medium + Chloramphenicol.

 Sandra use as negative control : 200µl plasmid (RBS+T) in medium with Amp and inoculate in LB medium with Chlorampheenicol. No colonies have to grow because not have resistance for chloramphenicol.

 Incubate at 370 C in shaker.