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From 2011.igem.org

July 13, 2011

We did:

1. Electrophoresis of the products of yesterday PCR and 3 digestions.

What we expect to know? If EcoR1 enzyme digest and in how much microliters is efficient, that with dilution the amplification be more effective.

Results: We had not results of PCR :( !

Apparently EcoR1 enzyme cut the plasmid, we see bands of approximately 2 kb and others of 1,2 kb. We think that the gen isn't in the plasmid because the weight desired es of 4,4 kb (4,441pb)

We have to do electrophoresis again for:

-Check if primers weren't degraded, Results: There aren't

-If gen is mutated:

Good news: Sample 6 of 10/30/11- mutated gen!

Bad news: rh1AB + psB1C3 of 10/30/11 isn't observed. We take DNA of this sample for transformation and because of that we had questionable results.