Team:Northwestern/Results/Data

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Figure 1: The diagram above shows an overview of our system demonstrating two of our main constructs.
fig1


Favorite Parts


  • [http://partsregistry.org/Part:BBa_K575024 BBa_K575024 Main Page] This construct consists of the gene for GFP, regulated by a promoter that is induced by the dimerization of lasR and 3-oxo-C12-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the lasR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of 3-oxo-C12-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green if and only if autoinducer is present. The response was not highly dose-dependent. This means that the lasR receptor protein is successfully being produced and the promoter induced by the lasR/3-oxo-C12-HSL complex is working properly.
  • [http://partsregistry.org/Part:BBa_K575033 BBa_K575033 Main Page] This construct consists of the gene for GFP, regulated by a promoter that is induced by the dimerization of rhlR and C4-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the rhlR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of C4-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green if and only if autoinducer is present. The response is dose-dependent. This means that the rhlR receptor protein is successfully being produced and the promoter induced by the rhlR/C4-HSL complex is working properly.
  • [http://partsregistry.org/Part:BBa_K575046 BBa_K575046 Main Page] This construct consists of the gene for GFP, regulated by a promoter (which we isolated from the Pseudomonas genome) that is induced by the dimerization of rhlR and C4-HSL (a signaling molecule produced by Pseudomonas Aeruginosa), as well as a constitutively expressed gene for the rhlR receptor protein. Cells transformed with this construct would ideally fluoresce green in the presence of C4-HSL, thereby indicating the presence of Pseudomonas Aeruginosa. We have shown that our cells do in fact fluoresce green if and only if autoinducer is present. The response is also dose-dependent. This means that the rhlR receptor protein is successfully being produced and this newly-isolated promoter induced by the rhlR/C4-HSL complex is working properly.


Well-characterized Parts


  • [http://partsregistry.org/Part:BBa_K575008 BBa_K575008 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575024 BBa_K575024].
  • [http://partsregistry.org/Part:BBa_K575009 BBa_K575009 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575028 BBa_K575028].
  • [http://partsregistry.org/Part:BBa_K575012 BBa_K575012 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575032 BBa_K575032].
  • [http://partsregistry.org/Part:BBa_K575013 BBa_K575013 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575036 BBa_K575036].
  • [http://partsregistry.org/Part:BBa_K575023 BBa_K575023 Main Page] This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the dimerization of rhlR and C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575028 BBa_K575028 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of 3-oxo-C12-HSL.
  • [http://partsregistry.org/Part:BBa_K575032 BBa_K575032 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575036 BBa_K575036 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575037 BBa_K575037 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575042 BBa_K575042 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575046 BBa_K575046].
  • [http://partsregistry.org/Part:BBa_K575043 BBa_K575043 Main Page] This construct successfully functioned as part of Part [http://partsregistry.org/Part:BBa_K575047 BBa_K575047].
  • [http://partsregistry.org/Part:BBa_K575047 BBa_K575047 Main Page] This construct enabled cells to physically convey the presence of Pseudomonas Aeruginosa by fluorescing green in the presence of C4-HSL.


Characterization of Pre-existing Parts


  • [http://partsregistry.org/Part:BBa_C0071:Experience BBa_C0171 Experience] This is the rhlR receptor protein without the LVA tag.
  • [http://partsregistry.org/Part:BBa_C0079:Experience BBa_C0179 Experience] This is the lasR receptor protein without the LVA tag.
  • [http://partsregistry.org/Part:BBa_R0071:Experience BBa_R0071 Experience] This is a promoter regulated by rhlR and C4-HSL.
  • [http://partsregistry.org/Part:BBa_R0079:Experience BBa_R0079 Experience] This is a promoter regulated by lasR and 3-oxo-C12-HSL.


Other Parts


  • [http://partsregistry.org/Part:BBa_K575004 BBa_K575004 Main Page] This construct was intended to continuously express the lasR receptor protein. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575005 BBa_K575005 Main Page] This construct was intended to continuously express the lasR receptor protein. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575006 BBa_K575006 Main Page] This construct was intended to continuously express the rhlR receptor protein. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575007 BBa_K575007 Main Page] This construct was intended to continuously express the rhlR receptor protein. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575010 BBa_K575010 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575011 BBa_K575011 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575014 BBa_K575014 Main Page] This construct was intended to produce RFP in the presence of rhlR and C4-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575015 BBa_K575015 Main Page] This construct was intended to produce RFP in the presence of rhlR and C4-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575020 BBa_K575020 Main Page] This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the dimerization of lasR and 3-oxo-C12-HSL.
  • [http://partsregistry.org/Part:BBa_K575021 BBa_K575021 Main Page] This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the dimerization of lasR and 3-oxo-C12-HSL.
  • [http://partsregistry.org/Part:BBa_K575022 BBa_K575022 Main Page] This is a promoter region extracted from the Pseudomonas Aeruginosa genome. It is activated by the dimerization of rhlR and C4-HSL.
  • [http://partsregistry.org/Part:BBa_K575025 BBa_K575025 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575026 BBa_K575026 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575027 BBa_K575027 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575029 BBa_K575029 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575030 BBa_K575030 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575031 BBa_K575031 Main Page] This construct was intended to produce RFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575035 BBa_K575035 Main Page] This construct was intended to produce RFP in the presence of rhlR and C4-HSL. This construct was also intended to constitutively express the rhlR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575038 BBa_K575038 Main Page] This construct was intended to produce RFP in the presence of rhlR and C4-HSL. This construct was also intended to constitutively express the rhlR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575039 BBa_K575039 Main Page] This construct was intended to produce RFP in the presence of rhlR and C4-HSL. This construct was also intended to constitutively express the rhlR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575040 BBa_K575040 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575041 BBa_K575041 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. Testing has not provided conclusive results.
  • [http://partsregistry.org/Part:BBa_K575044 BBa_K575044 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.
  • [http://partsregistry.org/Part:BBa_K575045 BBa_K575045 Main Page] This construct was intended to produce GFP in the presence of lasR and 3-oxo-C12-HSL. This construct was also intended to constitutively express the lasR receptor protein. Testing has not shown the desired results.


Sponsor northwestern.jpg Sponsor weinberg.jpg Sponsor mccormick.jpg
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