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Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8

Project: Regulatory

Date: Fri, 09 Sep 2011 12:13:01 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

• Fri, 09 Sep 2011 12:19:11 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)
• Fri, 09 Sep 2011 12:19:30 GMT (davis950): entry data updated by davis950 (id=2)

Purpose: to digest pUC-pcyA-ho1 and pUC-pTet-Cph8 with appropriate restriction enzymes to put pTet-Cph8 in pUC-pcyA-ho1.

Procedure:

The following digestion reactions were prepared:

• 16 ul ddH2O

• 1 ul SpeI

• 1 ul PstI

• 3 ul NE buffer 2

• 10 ul pUC-pcyA-ho1 miniprep product (139 ng/ul)

• 16 ul ddH2O

• 1 ul XbaI

• 1 ul PstI

• 3 ul NE buffer 2

• 10 ul pUC-pTet-Cph8 (105 ng/ul)

Digest reactions placed in 37 degree C incubator overnight.

Results: None

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