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From 2011.igem.org

7/8 - Advisor's presentation

Advisor’s presentation

• Mechanisms
• Plans
• Things needed to be ordered

-Vibrio harveyi; media check; KACC number check

• Things needed to be done by individual members

-Analyze trends of supporting data

-Detection methods by TLC, GC, HPLC, and understand the mechanism

-Understand reasons for codon optimization

• Things needed to be done as a group

-Group 1: Search for promoter and vector type

-Group 2: Search for C14’s usage; cost, etc.

-Group 3: Search for medium conditions

7/22 - Presentation on experiment

Group 1

• Presented about

1. Plasmid in which the gene will be incorporated

2. Promoter Tac = hybrid of E.coli's trp and lac operon

- Strong promoter of gene expression

3. Induction can be done by the addition of IPTG(isopropyl-beta-D-1-thiolgalactopyranoside)

- IPTG is a derivative of lactose and is used as an inducer to operate the promoter.

4. Repression can be done by the expression of lac1 protein, which is a type of repressor

- lac1 protein is a DNA-binding protein involved in the metabolism of lactose.

- Promoter mutation: intracellular concentration of lac1 repressor is increased, leading to stronger repression.