Team:ITESM Mexico/Parts submitted

From 2011.igem.org

ITESM MÉXICO

SensE.coli

Igem Itesm

Parts submitted


Part Registry


Name Description Type Designer Length Lenght (prefix;suffix)
BBa_K587000 RecA Protein coding Antonio Villarreal 1062 1103
BBa_K587001 Crxst Regulatory David Maycotte 35 78
BBa_K587002 Tast Regulatory David Maycotte 80 123
BBa_K587003 Tawk Regulatory David Maycotte 79 122
BBa_K587004 Crxwk Regulatory David Maycotte 35 78
BBa_K587005 Itast Regulatory David Maycotte 81 124


Sequences and description


  • [http://partsregistry.org/Part:BBa_K587001 RecA]
Protein coding sequence

Sequence

GAATTCGCGGCCGCTTCTAGATGGCTATCGACGAAAACAAACAGAAAGCGTTGGCGGCAGCACTGGGCCAGATT GAGAAACAATTTGGTAAAGGCTCCATCATGCGCCTGGGTGAAGACCGTTCCATGGATGTGGAAACCATCTCTACCGGTTCGCTTTCACTGGATAT CGCGCTTGGGGCAGGTGGTCTGCCGATGGGCCGTATCGTCGAAATCTACGGACCAGAATCTTCCGGTAAAACCACGCTGACGTTGCAGGTGATCGCCGC AGCGCAGCGCGAAGGTAAAACCTGTGCGTTTATCGATGCTGAACACGCGCTGGACCCAATCTACGCACGTAAACTGGGCGTCGATATTGACAACCTGCT GTGCTCCCAGCCGGATACCGGCGAGCAGGCACTGGAAATCTGTGATGCCCTGGCACGTTCTGGCGCAGTAGACGTTATCGTCGTTGACTCCGTGGCGGCAC TGACGCCGAAAGCGGAAATCGAAGGCGAAATCGGCGACTCTCACATGGGCCTTGCGGCACGTATGATGAGCCAGGCGATGCGTAAGCTGGCGGGTAACCTG AAGCAGTCCAACACGCTGCTGATCTTCATCAACCAGATCCGTATGAAAATTGGTGTGATGTTCGGTAACCCGGAAACCACTACCGGTGGTAACGCGCTGAAA TTCTACGCCTCTGTTCGTCTCGACATCCGTCGTATCGGCGCGGTGAAAGAGGGCGAAAACGTGGTGGGTAGCGAAACCCGCGTGAAAGTGGTGAAGAACAAA ATCGCTGCGCCGTTTAAACAGGCTGAGTTCCAGATCCTCTACGGCGAAGGTATCAACTTCTACGGCGAGCTGGTTGACCTGGGCGTGAAAGAGAAGCTTATC GAGAAAGCAGGCGCGTGGTACAGCTACAAAGGTGAGAAGATCGGTCAGGGTAAAGCGAATGCGACTGCCTGGCTGAAAGATAACCCGGAAACCGCGAAAGAG ATCGAGAAGAAAGTACGTGAGTTGCTGCTGAGCAACCCGAACTCAACGCCGGATTTCTCTGTAGATGACAGCGAAGGCGTAGCAGAAACTAACGAAGATTT TTAATACTAGTAGCGGCCGCTGCAG


  • [http://partsregistry.org/Part:BBa_K587002 Crxst]
This DNA sequence acts as a lock for the low arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTTGGATTTGGGTATCATACTAGTAGCGGCCGCTGCAG


  • [http://partsregistry.org/Part:BBa_K587003 Tast]
This sequence acts as the key for the Crxst lock and it unlocks it, in order to activate following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGAACCTAAAGGGATAGTT TACTAGTAGCGGCCGCTGCAG


  • [http://partsregistry.org/Part:BBa_K587004 Tawk]
This sequence acts as the key fot Crxwk lock and it unlocks it, in order to activate the following mechanism.

Sequence

GAATTCGCGGCCGCTTCTAGAGAGATCTCTATATACCATACCACCAATTAAAAAGTAATTGGTGGTTTCGCGTCATTAAGTGGAGATAAACCGTAAGGGTT TACTAGTAGCGGCCGCTGCAG


  • [http://partsregistry.org/Part:BBa_K587005 Crxwk]
This DNA sequence acts as a lock for the high arabinose concentration regulation path.

Sequence

GAATTCGCGGCCGCTTCTAGAGAAGCGCAGTAATTCACCTCTATTTGGCATTCCCAATACTAGTAGCGGCCGCTGCAG


  • [http://partsregistry.org/Part:BBa_K587006 Itast]
This is a complementary sequence for the Tast key, so we call it the antikey since it will inactivate the Tast key and preventing it to interact with the Crxst lock.

Sequence

GAATTCGCGGCCGCTTCTAGAGTCTAGGAGAGATATATGGTATGGTGGTTAATTTTCATTAACCACCAAAGCGCAGTAATTCACCTCTTGGTTTCCCTATCAA TACTAGTAGCGGCCGCTGCAG