Ligation of promotor, RBS in vector
Investigators: Sophie, Theo, Sandra
Comments:For ligation digested samples were used from yesterday.
Every promotor was ligated with every RBS using the Ginko Bioworks 3A-assembly protocol.
PR1: S1 with S4 (660bp)
PR2: S1 with S5 (1000bp)
PR3: S1 with S6 (426bp)
PR4: S2 with S4 (233bp)
PR5: S2 with S5 (940bp)
PR6: S2 with S6 (981bp)
PR7: S3 with S4
PR8: S3 with S5
PR9: S3 with S6
Name for each construct:
|
| sample
| name
|
| Promotor
| BBa_J23104
| S1
|
| Promotor
| BBa_J23110
| S2
|
| Promotor
| BBa_J23116
| S3
|
| RBS
| BBa_B0034
| S4
|
| RBS
| BBa_B0032
| S5
|
| RBS
| BBa_B0031
| S6
|
| Vector
| pSB1C3
| S7
|
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