Team:Calgary/Notebook/Protocols/Process20
From 2011.igem.org
Preparing Glycerol Stocks
Reagents and Materials
- Overnight bacterial growth
- Screw Cap Tubes
- Glycerol
Protocol
- Pipet 0.5mL of 50% glycerol into 3 1.5mL screw cap tubes
- Add 0.5mL of overnight culture to each tube.
- Pipet up and down to gently mix.
- Flash freeze in liquid N2 or dry ice/ethanol bath.
- Place in -80°C freezer when frozen.