Team:Peking S/lab/notebook/lwh

Wenhan Luo's Notebook

summary

My major task is molecule cloning of generators and corresponding receivers for chemical wires such as A-factor. In addition, characterization of some logic gates like AND GATE as well as constructing a three input gate using new signaling molecules is my major contribution in the later stage of our poject. What is more, I am also responsible for the direction and manufacture of the CG animation about invertors.

Contents

6.24-7.5 Learn the basic experiment skills including transformation and ligation and digestion.

7.6-7.7 Ask professor Honglong some questions about Streptomyces and acquire the bacteria strain from him.

7.8-7.12 Try to PCR an a-factor generator gene(afsa) and its corresponding receivers, the repessor gene called arpa from Streptomyces.

7.12-7.16 Using different enzymes(prime star、esay pfu、TAG ) in PCR to amplification afsa gene and arpa gene from the Streptomyces .

7.17-7.23 No matter which DNA polymerase we used,we can not obtain those two genes, so we try to change the anneal temperature in PCR process.

7.24-7.28 I got afsa and arpa eventually and going to construct a-factor chemical-wire gene circuit.

7.29-8.18 Constuct a three input logic gate whose input is CAI-I and Arabinose and Salicylate and output is green fluorescence protein.

8.19-9.19 Do a ribosome binding site mutation of an AND GATE whose input is AHL and Salicylate.

9.20-10.1 Make a characterization of the salicylate(sal), induce a gene circuit which consists of the sal promoter and a gfp gene by different concentration and different time span.Get the fluorescence intensity cell by cell using FCM(flow cytometry) and depict the graph.