07.21-07.28
Construted Terminator-pBAD promoter for part assembly in the next step.
07.29-08.05
Attended iGEM 2011 China Meetup and had a few days’ holiday.
08.06-08.13
Characterize deoxyviolacein primarily by TLC (developed by dichloromethane: methanol = 6:1 (v/v)) and spectrophotometry (absorbance wavelength=570nm).
Constructed Terminator(B0015)-pBAD-vioABD.
08.14-08.18
Constructed Terminator(B0015)-pBAD-vioC with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioCC”. Sequencing results showed that it is actually Terminator(B0015)-pBAD-vioD with scores of base pair from vioB at 5’ and scores of base pair from vioE, termed “vioDD”.
Constructed Terminator(B0015)-pBAD-vioE.
08.19-08.25
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioE & Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, both of whose ribosomal binding sites were mutated to AGGAGGT.
08.26-08.31
Constructed Terminator(B0015)-pBAD-vioABDE.
09.01-09.08
Constructed Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, whose ribosomal binding site is native.
09.09-09.15
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD.
Constructed Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE, in the backbone of pSB3K3.
09.16-09.23
Acquired HPLC results of the extraction from E. coli producing proviolacein and deoxychromoviridans.
Constructed Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD-Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE & Terminator(B0015)-pBAD-TPP ribozyme 1.20-vioD -Terminator(B0015) -pBAD-vioE.
09.24-10.04
Constructed Terminator(B0015)-pBAD-vioAB- Terminator(B0015)-pBAD- TPP ribozyme 1.20-vioD- Terminator(B0015) -pBAD-vioE.
Switched the backbone of Terminator(B0015)-pBAD-vioABD- Terminator(B0015)-pBAD-TPP ribozyme 2.5-vioE to pSB1C3.
Part submission.
Wiki editing, part documentation. |
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