07.01-07.07
I practiced the skills of molecular biology experiments such as PCR, transformation, plasmid extraction and anticipated in the preparation for the genetic selection with my partner Xiao Qingyang.
I designed the construction of the wiki and learned how to use some softwares, for instance, Dreamweaver, Photoshops, Flash to do our wiki.
07.8-07.14
We selected(ON) the Top 9 in M9 culture. But here something went wrong that the bacteria could not suvive in the M9(amp.) culture. So we adjusted the protocols and changed the bacteria with Top 10, which contributed to the survive of the bacteria through the progress of ON Selection.
With the help of Zheng Luo, I was able to get plenty of picture ingredients for our wiki. I designed our mainpage flash and banners of every pages, and so was the two navigation bars.
07.15-07.21
We did the off-selection with NiCl2.
Luckly, I finished the pages of biosafety and project description before the deadline. Then, I designed parts of pages within the Team section.
07.22-07.28
We found something wrong in our culture that we were not supposed to add glucose to our culture. So we restarted our selection again. At the same time, I checked that the AND GATE BBa K228258 made by Team Peking in 2009 did work.
In addition, I made our team gallery on our wiki.
07.29-07.31
Haoqian Zhang, Shuobin Chen, Zherun Zhang, Xiaowei Yan and I went to USTC to attend to the 2011 China Meetup held in Hefei. We gave a presentation and made many friends there.
08.01-08.04
I got another task with YanGong and Yangyang Zhao to add the “tpp” to the biostable backbond.
I took some work about Human Practice, we designed a survey, printed them and do the survey. I also added this part on our wiki.
08.05-08.12
We handed out our survey in our biology building, collected them, gathered and analyzed Data. I also amplified the Tpp and pBAD Biostable in PCR, cut them with restriction enzyme and link them. Then I transformed the produce into Trans 5ɑ(one kind of competent cells from Escherichia coli) Unfortunately, there was no bacteria survive on the plate.
08.13-08.15
I did the same experiment I did these days in the Lab again, and failed again.
08.30-09.05
I designed the Notebook Section on our wiki, and improvesd several webs of our wiki.
09.06-09.15
I finished the Team Section on our wiki.
09.15-09.30
I finished the Human Practice Section, and designed the picture for our parts. I also improved our wiki style.
10.01-10.04
I drew the pictures of our project and sent the text on our wiki. |
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