From 2011.igem.org
PCR Overlap Extension
Project: Surface Display
Date: Thu, 08 Sep 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Fri, 16 Sep 2011 21:59:17 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Although the previous PCR Extension worked, one of the postdocs suggested that the primers may interfere with the next step of the extension so the reaction was repeated.
Procedures:
The PCR reaction was done with phusion.
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