From 2011.igem.org
Ligation of pBAD and OmpA
Project: Surface Display
Date: Sat, 23 Apr 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 22 Aug 2011 10:19:31 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Ligate the digested pieces together.
Procedure:
Using the following equation to calculate the ratio of insert and plasmid needed.
((ng vector x kb insert)/(kb vector))x(mols insert/mols vector)=ng insert.
pBad=4.2 kb
OmpA=1 kb
Results:
Unknown since the lab was locked.
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