From 2011.igem.org
Digestion
Project: Surface Display
Date: Tue, 16 Aug 2011 18:14:41 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 18:16:58 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
Objective: double digest INP & pMCS5BB
INP tube
- 1 uL BamHI
- 1 uL XhoI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 uL INP DNA
pMCS5BB tube
- 1 uL BamHI
- 1 uL XhoI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 plasmid DNA
Incubate at 37 degrees overnight
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