Team:UT Dallas/week3

Incubate in 3 mL of LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
Ligation of Photo and YFP (i2.5.4 and i2.5.1) for 10 minutes at room temperature
Transformation of Photo and YFP 50 µL DH5a, 2 µL DNA at 37 degrees Celsius overnight (all chlora)
Making competent cells- 20 tubes (250 µL each) RP437, 20 tubes (250 µL each) BW2513

7/21/2011

Glycerol stock- 150 µL of 50% glycerol and 350 µL of cells
Miniprep
Incubate in 3 mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm
Test digestion for 1 hour and 20 minutes a 37 degrees Celsius

7/22/2011

Glycerol Stock- 150 µL of 50% glycerol and 350 µL of cells
Miniprep
Glycerol stock- 350 µL of cells abd 150 µL of 50% glycerol
Dissolving and Diluting primers