Team:UT Dallas/week1

Week 1

7/11/2011	Preparing sent parts

7/12/2011	Preparing sent parts growing LB and appropriate antibiotic overnight

7/13/2011	all grew, negative controls didn't Preparing sent parts, take 200µL from LB grown overnight and plated on appropriate antibiotic

7/14/2011	all grew a blob and a few colonies along the edge Transformation at 37 degrees Celsius overnight- 50 µL DH5a, 2 µL DNA (all carb) Plating sent parts Take one colony from July 13 plate properly

7/15/2011	Transformation of 50µL of DH5a, 2 µL DNA (all carb) at 37 degrees overnight Incubated in 3mL LB and appropriate antibiotic overnight at 37 degrees Celsius and 220 rpm.