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From 2011.igem.org

This page is the note of Luminescence Group.

Contents 1 Aug 4th, 2011 2 Aug 5th, 2011 3 Aug 6th, 2011 4 Aug 7th, 2011 5 Aug 8th, 2011 6 Aug 9th, 2011 7 Aug 11th, 2011 8 Aug 12th, 2011 9 Aug 17th, 2011 10 Aug 18th, 2011 11 Aug 19th, 2011 12 Aug 22th, 2011

Aug 4th, 2011

• We got drosophila melanogaster(oregon-rs) in 3 tubes from [http://gcoe.biol.sci.kyoto-u.ac.jp/gcoe/eng/member/2008/07/fuse_naoyuki.php Mr. Fuse].
• We made [http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html standard medium of Tokyo Metropolitan Univ. for drosophila].

[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Standard medium of Tokyo Metropolitan Univ. for drosophila]

Materials	Methods
water 500mL dry yeast 20g corn flour 45g glucose 50g agarose 3.5g propionic acid 1.5mL	Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it. Stir corn flour and glucose with the remaining water. Stir 1 and 2, then autoclave it again. after autoclave, add propionic acid into it.■

• We dispensed the standard-medium into 3 bottles.
• Kusaba made a convenient tube for taking flies.

Aug 5th, 2011

• When we transfer flies to bottle No.4, we sadly found most of they drown. Then we wipe off the water drops inside the bottles and use dry heat sterilizer to dry them. The problem is solved and we dispense flies to No.5 and No.6, from No.4 and No.2 respectively.
• An extra 0.3g agarose is added into the 100ml standard-medium to make the solid-medium. We clean the bottle No.4 and dispense the solid-medium into it and labeled No.7.
• We practiced ice anesthesia.

Aug 6th, 2011

• After drying lightly the bottle No.7, we transfer the flies from No.3 into it.

Aug 7th, 2011

• We dispensed standard-medium to six bottles and solid-medium to another two bottles.

Aug 8th, 2011

• Not enough flies to dispense.
• The experimental plan was made by Kusaba.

Aug 9th, 2011

• Transfer part of the flies from No.6 to No.8(standard-medium).
• Supplement the standard-medium in No.1~3.

Aug 11th, 2011

• Transfer flies from No.5 to No.9(standard-medium).

Aug 12th, 2011

• Transfer the rest of the flies from No.6 to No.10(standard-medium).

Aug 17th, 2011

• We transfer the second generation of flies as follows:
  • No.1 -> No.11
  • No.2 -> No.12
  • No.3 -> No.13
  • No.5 -> No.14
  • No.6 -> No.15
• We dispose the imaginal flies of No.7.

Aug 18th, 2011

• Our group assembled the first experimental system to research the phototaxis of flies. However, the response of flies was not clear and we could not get respectable results.
• Fly dispension:
  • No.12 -> No.14
  • No.3, 5, 6, 7 -> No.16
  • No.1, 2 -> No.17
• We made the medium with a little change as follows:
  • agarose 3.5g -> 4.0g
  • Add "Bokinin" 5 ml(p-Hydroxybenzoate 0.5 g in 5 ml of 70% alcohol solution)

Aug 19th, 2011

• Fly dispension:
  • No.11 -> No.18(second generation).
  • No.5, 6, 7 ->No.19
  • No.2, 3 ->No.20
  • No.1 -> refrigerator
  • No.8 -> No.9

Aug 22th, 2011