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From 2011.igem.org

Notebook

Week 1: May 16-20

Monday

Today we laid out the weekly schedule for assembly, as follows

Day 1: grow culture

Day 2: Assemble (all day)

Day 3: grow colonies and check with PCR

Day 4: miniprep

Day 5: sequence results

Day 6: use miniprep

Note: Every step is done each day for different assemblies

We also began developing preliminary ideas for the summer project:

Proposed Idea	Preliminary Thoughts
Bacterial photosynthesis	Up-regulate bacterial chlorophyll, BCHM=gene to increase chlorophyll production
Microbial fuel cells	Bacteria add electrons to Fe+3 and electrodes to create current, Use sacrificial anode (geobacter)
Serotonin sensor	????
Bacterial arithmetic	Use sRNAs to regulate translation to mirror arithmetic operations based on expression rates

We spent the rest of the day researching these ideas to determine their plausibility.

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