Team:Missouri Miners/Notebook

*Resuspension
 of Omp-R, 
 eYFP, and RBS

 7/29 Details

*Isolated
 plasmids
           

*Mini Preps.
*Digest1    
           

*Ran Gel for
 Digest1   
 (Gel Incon-
 clusive)      

*Re-did Digest1
 and Gel1      
*Cut out bands 

*Ectracted    
 plasmid parts
 from gel
*Ligation1     

*Transformation
 of E.coli with
 lig.1 products
*Digest2 (eYFP
 and RBS)
*Gel of Digest2
*Extracted bands
 of Digest2

*Transform.1
 had colonies   
*Re-did eYFP 
 digest2
*Ran eYFP D2 gel
*Extracted eYFP
*Ligation2

*Transformation2
*Innoculated    
 transform.1 into
 broth

*Isolated plasmids
 from transform.1