Team:Caltech/Week 12

August 28

Made R0010-MG1655 and J23119-MG1655 plates and overnights Continued growing biofilm in flask with glass beads; added fresh amphicillin Mixed more X-gal and IPTG solution at 20mg/mL and .1M concentrations respectively Attempted to induce R0010 overnights with IPTG to cleave X-gal; J23119 to cleave X-gal without inducing

Results

J23119-MG1655 grew, but the R0010-MG1655 did not R0010 did not cleave X-gal even with a high concentration of both IPTG and X-gal J23119 cultures turned blue with cleaved X-gal after about 4 minutes

August 29

R0010 overnight culture did not grow Redo p450 experiments in MeOH in order to send to electrospray Attempt to measure J23119-X-gal reaction with spectrophotometer

Results

J23119 culture was too thick and not in exponential growth phase; redo

August 30

HPLC of yesterday's p450 reaction and purification Overnights of DDT-pET, WT-F87A, and lacZ colonies for sequencing, BioBrick submission, spectrophotometer experiments, etc. Make M9 media for suspending X-gal for biofilm column experiments

Results

HPLC results revealed too much noise; chemicals at too low of concentration