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From 2011.igem.org

Monday

Labwork

• PCR purification of B1 after prefix-suffix-PCR. The purifactions are Nanodropped - B1 samples 3,4,5,8 and 10 looks fine.

• Digestions (1 h by 37 degrees or O/N)

* PSB1C3 with E + P
* A2 in PSB1C3 with X + P
* Expr.vector with P + S
* B1 3,4,5,7 and 10 with E + P

• Purification of the digestions by using the PCR purifaction kit

• Ligations

* PSB1C3 + B1 samples 3,4,5,8 and 10
* Expr. vector + A2
* Selfmade Expr. vector + A2

• Transformation of ligations

• Made O/N cultures on: 2C9, UserB1 to Sequencing, UserB1 to growth and Control BL21 cells to growth.

• Made TB media and autoclaved bottles for tomorrow.

• Made Cam + IPTG plates

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