Team:NTNU Trondheim/Stress Sensor

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Stress sensor (rrnB+LacI+pLac+mCherry)

RrnB+LacI+pLac+mCherry rosa.png


The stress sensor is our main construct. It is made from the following biobricks:

Plasmid map of the complete construct. Promoters are shown in blue, and genes as inside arrows. Only single restriction sites are shown.
Biobrick Part number
LacI with RBS BBa_J24679
Terminator BBa_B0015
pLac BBa_R0011
mCherry with RBS and terminator BBa_J06702

The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)


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