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Protocols

Fundamental protocols ::: These protocols are necessary for all lab operations. As many of the subjects are general, the protocols may contain few specific steps.

Autoclave usage

OD600 optimization

Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.

Production of electrocompetent cells

Electroporation of electrocompetent cells

Production of standard competent cells

Quick Transformation (QT) of standard competent cells

Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.