Team:NTNU Trondheim/Stress Sensor

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    • LacI+pLac +mCherry
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Stress sensor (rrnB+LacI+pLac+mCherry)

The stress sensor is our main construct. It is made from the following biobricks:

Biobrick	Part number
LacI with RBS	BBa_J24679
Terminator	BBa_B0015
pLac	BBa_R0011
mCherry with RBS and terminator	BBa_J06702

The promotor is a variant of BBa_K112118, that we have made ourselves using PCR. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)