Team:KULeuven/Figures

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FIGURES

Figure 1

Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K₁= terminator, K₂= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L

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Figure 2

Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator.

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Figure 3

Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor.

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Figure 4

Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, H₂= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 3₁= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 3₁= Mnt repressor, H₁= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI.

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Figure 5

Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor.

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Figure 6

Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).

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Figure 7

Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).

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Figure 8

Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI.

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Figure 9

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