Sunday, August 21

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Please tell the hours, both coming and going, that you would like to be working in the lab today. If none, please say so.

Patrick O' Neill to open Lab at 2:00 today.

Jessica Hobson 2 p.m.-6:30 p.m.

Dr Liz I'll be available for phone consulting from 2:00 - 6:00+ @ 443.610.5259. I'm very glad Patrick can open the lab today as I'm not feeling well (on antibiotic since yesterday) and have much paperwork still to finish re: iGEM's use of Smith 491. l will be working from home all day unless I begin to feel much better.

Steve Stowell I'll be in 2:30 to 3:00, leaving about 7:30, or a convenient stopping point.

Prepare two gels to be stored in 491 refrigerator

2% GelPilot LE Agarose

100 mL TAE 1X Buffer

2g GelPilot LE Agarose (1,000-23,000bp)

Prepare 10 uL PCR product with 2 uL 6x Blue loading dye to be stored in 491 freezer. These are the colony PCR products that were amplified on 8/18/11 from the transformations on 8/4/11.

3B Undiluted: 1

4A 50%transformation: 1,2,3,4,5,6,7,8,9,10,11,12,13

4A Undiluted: 1,2,3

positive control

negative control

Next lab samples need to be loaded into the gels and ran. Transformation of 5I and 5K showed no growth. These transformations should be redone next lab.

Patrick and Jessica 2-6:30p.m. Steve 3-6:30p.m.