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Team:Michigan/Notebook
From 2011.igem.org
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Calendar of Events
Protocols
Notebook
5/13/2011
Kevin, Ben, Candy, Brian, Alison
⇒ Unpacked supplies from last year
⇒ Restocked lab and created inventory
⇒ Training protocols written
5/19/2011
Kevin, Alena, Ben, Namun
⇒ PCR machine is now operable. Basic programs have been written, but not all the functions of the machine are known. No protocol for using the PCR machine has been written.
⇒ Usage of the autoclave was determined. No protocol exists for autoclaving yet.
⇒ 25 plates were made using 10g LB agar stock, 250mL deionized water, and 250μL ampicillin from vials left over in the stupid white box in the freezer. Original protocol called for distilled water-abbreviation "dH2O" was misinterpreted to mean deionized. Not known if the plates are viable due to this error. House-made protocols will use the following abbreviations to avoid this confusion:
∴Distilled water: dH2O
∴Double distilled water (from the machine): ddH2O
∴Deionized water: DI water or diH2O
A protocol has been written for plate production. As it depends on the autoclave protocol, however, it cannot be considered complete.
⇒ Simple inventorying took place, with the locations of pipettes, ethanol, hardware, and the master gas shutoff(!) determined.
⇒ Inventorying will continue tomorrow, and a PCR reaction may be attempted. Protocols for the PCR purification kit (Miniprep) will be modified during this time, assuming the PCR reaction proceeds. No method currently exists to determine PCR outcome, as no gels or gel mix is available in the lab. Along these lines, it may be beneficial to find an ultraviolet spectrophotometer to run these tests.
5/20/2011
Amy, Ben, Candi
⇒ Cells were transformed with extra 173 pGLO vector in accordance with the QT protocol. This used stock competent cells found in the refrigerator. Protocol modifications will be made accordingly.
⇒ Access to room 3152 is crucial, and no means of entry currently exists. This will be imperative if we require ddH2O, an autoclave, LB stock, a normal scale, large glassware, or any number of other items.
⇒ Due to lack of access to 3152, protocols requiring sterile water cannot be tested. Sterile water exists in 3151A, but is 3 years past its expiration date.
⇒ No source of agarose or ethidium bromide has been found, so electrophoresis protocols cannot be tested. Interestingly, there exists plenty of loading dye.
⇒ Large amounts of stock compounds IE ethanol belong to the room and are not owned by us. Currently they are being used. This may cause problems, especially if we start using more expensive materials we still don't own.
On a side note, the contrast between the physical sign-in sheet in 3151 and the online form on ctools is rather confusing-it may be better to just use the online one, and have people log their hours individually.
5/22/2011
Ben ⇒ Two containers of 500mL LB broth were produced and autoclaved. 12.5g of LB powder was combined with 500mL water for each container.
5/24/2011
Ben ⇒ Six Erlenmeyer flasks of diH2O were autoclaved and are now sterile. Both sets of glassware reside on the shelf next to the incubator.
