"

From 2011.igem.org

Week 1: 20.6-26.6

• First meeting

• Brainstorming

Week 2: 27.6-3.7

• Brainstorming

Week 3: 4.7-10.7

• Design of several Operons for AlcR

Week 4: 11.7-17.7

• Cloning of the
  • AlcR-testsystem

Week 5: 18.7-24.7

• First test of the AlcR-system in 96-well plates
• Transformation of the parts from the iGEM plates

Week 6: 25.7-31.7

Week 7: 1.8-7.8

• Growth test of DH5-α with AlcR-system in flasks

• Ligation and Transformation of
  • λ_P and luxI
  • λ_P and lacI
  • P_lac and GFP_LVA
  • P_lux and mCherry
  • P_tet and CI
  • P_const and luxR

Week 8: 8.8-14.8

Synthesized LacI_M1 Gen arrived

• Ligation and Transformation of
  • P_lac-GFP_LVA and Terminator
  • P_lux-mCherry and Terminator
  • P_const-luxR and Terminator

• Exchange of same rare codons in AlcR by PCR

• Transformation of λ_P

Week 9: 15.8-21.8

Third test of AlcR-system with M9-medium

Week 10: 22.8-28.8