Lysis cassette
Digestion of Quickchange
| Name: Theo
| Date: 23.07.2011
|
| Continue from Experiment 22.07.2011
Quickchange V.3 with correct Lysis Template
|
| Project Name: Quickchange V.3 with correct Lysis Template
|
| 5μl
| Buffer, NEB4
|
|
|
| 5μl
| BSA (10x)
|
|
|
| 2,5 μl
| Enzym 1
| DpnI
|
|
| 37,5 μl
| DNA
|
| Quickchange PCR
|
50 μl total volume
Incubate for about 1h at 37°C + Heat inactivation at 80°C for 20min.
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