Team:Freiburg/Notebook/14 July

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This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!


Contents

red light receptor

3A-assembly: Digest

Investigators: Jakob

3A-assembly of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator)

S21a:

  • Enzyme 1: XbaI
  • Enzyme 2: PstI

S22, S23

  • Enzyme 1: EcoRI
  • Enzyme 2: SpeI

Vector:

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI
  • Enzyme 3: DpnI


3A-assembly: Ligation and transformation

Investigators: Julia

Ligation of S22 (ho1)+S21a (terminator) and S23 (pcyA)+S21a (terminator) in vector pSB1T3.

L23: Ligation of S23+S21a in pSB1T3 L22: Ligation of S22+S21a in pSB1T3

Plasmids were transformed in cells and plated out.


PCR

Investigators: Julia

PCR of the red light receptor, cph8.

Primer:

  • P 10
  • P 11

DNA template:

  • BBa_I150010



Lysis cassette

Precipitator

Test-digest

Investigators: Rüdiger

Testdigest of the minipreps, Sophie did (june, 13).

  • Enzyme 1: PstI
  • Enzyme 2: EcoRI


caption

Image of different samples from miniprep.

                       

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