Team:Colombia/Notebook

From 2011.igem.org

Revision as of 04:23, 16 August 2011 by Olguitapr (Talk | contribs)

HeadCol.jpg

Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety Attributions

Contents

Daily iGemWork

Here you can find our daily work in the Lab!

June

June 30:

  • Biobricks 1, 2, 3, 4 and 5 were resuspended
  • Miniprep Solutions (I, II and III) were prepared.

July

July 1

  • Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

  • Biobrick 2 presented no colonies.
  • Colonies from bricks 1, 3, 4 and 5 were stinged.
     Task: 
     To make LB medium (15x25mL)

July 6

  • Biobricks 1, 3 and 4 were twice plated.
  • Brick 5 didn't grow up.
  • We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
  • LB medium was prepared (15x25mL).
  • Liquid LB was prepared (400mL).
     Task: 
     To add ampiciline and tetracicline to the boxes.
     Claim the liquid LB
     Scrape ....
     Sting biobricks 2 and 5 (no clons)
     Pick up the tubes with Merceditas

July 7

  • Clons 2 and 5 didn't work out.
  • Clons 1, 3 and 4 were planted in LB liquid.
  • E. Coli inoculation in Coffee
  • Kanamicine resistence plasmid: 0.2 optic density.
  • Direct inoculation x 2 and C(-) MgCl2.
     Task: 
     Print electroporation protocol.
     Ask Juan D. Olarte about the inoculation in coffee.
     Minipreps for confirmation of 1, 3 and 4.
     Competent cells for clones 2 and 5.

July 8

  • Minipreps for 1, 3 and 4 were made.
  • Plant sheets of coffee into different plates: Each sheet was cut in the middle and they were incubated at 25°C and 37°C LB+Kan.
     Task:
     To finish the minipreps from the addition of RNAsa.
     Check the E. Coli growth in coffee.

July 11

  • Minipreps have been finished. They've been planted in gel: Low concentration (25 ng/uL).
  • Transformation protocol in chemical cells.
     Task:
     Prepare 250 mL of SOC medium

July 12

August