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Contents 1 Daily iGemWork 1.1 June 1.1.1 June 30: 1.2 July 1.2.1 July 1 1.2.2 July 5 1.2.3 July 6 1.2.4 July 7 1.2.5 July 8

Daily iGemWork

Here you can find our daily work in the Lab!

June

June 30:

• Biobricks 1, 2, 3, 4 and 5 were resuspended
• Miniprep Solutions (I, II and III) were prepared.

July

July 1

• Biobricks 1, 2, 3, 4 and 5 were resuspended.

July 5

• Biobrick 2 presented no colonies.
• Colonies from bricks 1, 3, 4 and 5 were stinged.

     Task: 
     To make LB medium (15x25mL)

July 6

• Biobricks 1, 3 and 4 were twice plated.
• Brick 5 didn't grow up.
• We've added Ampiciline (3.75mL) and Kanamicine (1.875mL) to the boxed from the previous day.
• LB medium was prepared (15x25mL).
• Liquid LB was prepared (400mL).

     Task: 
     To add ampiciline and tetracicline to the boxes.
     Claim the liquid LB
     Scrape ....
     Sting biobricks 2 and 5 (no clons)
     Pick up the tubes with Merceditas

July 7

• Clons 2 and 5 didn't work out.
• Clons 1, 3 and 4 were planted in LB liquid.
• E. Coli inoculation in Coffee
• Kanamicine resistence plasmid: 0.2 optic density.
• Direct inoculation x 2 and C(-) MgCl2.

     Task: 
     Print electroporation protocol.
     Ask Juan D. Olarte about the inoculation in coffee.
     Minipreps for confirmation of 1, 3 and 4.
     Competent cells for clones 2 and 5.

July 8

• Minipreps for 1, 3 and 4 were made.