green light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
blue light receptor
NAME OF YOUR EXPERIMENT
Investigators:NAME
red light receptor
PCR for Cph8
Investigators:Julia
after protocol from Uppsala.
PCR-Mixture for one Reaction:
For a 50 µl reaction use
32,5µl
| H20
|
|
10µl
| 5x Phusion Buffer
|
|
2.5µl
| Primer fw
|
|
2.5µl
| Primer dw
|
|
1µl
| dNTPs
|
|
1µl
| DNA-Template
|
|
0.5 µl
| Phusion (add in the end)
|
|
Primer used:
cph8 Prefix: TTCGAATTCGCGGCCGCTTCTAGATGGCCACCACCGTACAA
cph8 Suffix: CCGCTACTAGTATTATTACCCTTCTTTTGTCATGCCCT
Lysis cassette
NAME OF YOUR EXPERIMENT
Investigators:NAME
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME