Team:Paris Liliane Bettencourt/Notebook/2011/08/13/

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Revision as of 11:31, 13 August 2011 by Cyrpaut (Talk | contribs)

Cyrille

Adding the RFP biobrick in pHM3

We want to insert the biobrick J04450 into pHM3 for three reasons:

  • To have a negative control of digestion
  • To restore the EXSP biobrick format in the plasmid
  • To test the possibility of cloning in this plasmid

3 times 500 ng of the pHM3 plasmid is digested in E P for 30 min

3 times 500ng of J04450 in pSB1C3 is digested using E P sites.

The result is loaded on a gel. The and will undergo a gel purification.

Insert digested before cutting the bands
Insert digested after cutting the bands
plasmid digested before cutting the bands
plasmid digested after cutting the bands
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