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From 2011.igem.org

Cyrille

Adding the RFP biobrick in pHM3

We want to insert the biobrick J04450 into pHM3 for three reasons:

• To have a negative control of digestion
• To restore the EXSP biobrick format in the plasmid
• To test the possibility of cloning in this plasmid

3 times 500 ng of the pHM3 plasmid is digested in E P for two hours using fast digest enzymes. As the fragment is short, they underdo a PCR purification to get rid of the small 43 bp interrior fragment.

3 times 500ng of J04450 in pSB1C3 is digested using E P sites. The result is loaded on a gel and will undergo a gel purification.