1. Thaw chemically competent cells on ice. While thawing, prepare DNA from well plates:
   • With a pipette tip, punch a hole through the foil cover into the corresponding well to the part that you want. Make sure you have properly oriented the plate (label rows/columns with a sharpie if it's the first time using the plate). Transfer excess DNA from well to a microcentrifuge tube and store at -20 °C.
   • Add 10uL of nuclease free H2O. Pipette up and down a few times and let sit for 5 minutes to make sure the dried DNA is fully resuspended.
2. Pipette 25uL aliquots of cells into tubes on ice. Add 1 uL DNA, then pipette gently to mix. Let sit for at least 30 minutes on ice.
3. Incubate cells for 40 seconds at 42 °C (use a stopwatch for exact timing).
4. Incubate cells on ice for 2 min.
5. Add 200 uL LB.
6. Incubate for 1 hour at 37 °C in shaker. If you need to cut corners, for Amp you can incubate for 5-10 minutes if you are in a hurry. For other ABs, can probably get away with about 40 minutes).
7. Spread 200uL onto a plate made with appropriate antibiotic.
8. Grow overnight at 37 °C.

Adapted from [http://openwetware.org/wiki/Transforming_chemically_competent_cells OpenWetWare] and [http://partsregistry.org/Help:Spring_2011_DNA_distribution The Parts Registry]