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From 2011.igem.org

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Calendar of Events

Notebook

5/13/2011

Kevin, Ben, Candy, Brian, Alison

Inventoried lab, worked on protocols

5/19/2011

Kevin, Alena, Ben, Namun

⇒ PCR machine is now operable. Basic programs have been written, but not all the functions of the machine are known. No protocol for using the PCR machine has been written.

⇒ Usage of the autoclave was determined. No protocol exists for autoclaving yet.

⇒ 25 plates were made using 10g LB agar stock, 250mL deionized water, and 250μL ampicillin from vials left over in the stupid white box in the freezer. Original protocol called for distilled water-abbreviation "dH2O" was misinterpreted to mean deionized. Not known if the plates are viable due to this error. House-made protocols will use the following abbreviations to avoid this confusion:

∴Distilled water: dH2O

∴Double distilled water (from the machine): ddH2O

∴Deionized water: DI water or diH2O

A protocol has been written for plate production. As it depends on the autoclave protocol, however, it cannot be considered complete.

⇒ Simple inventorying took place, with the locations of pipettes, ethanol, hardware, and the master gas shutoff(!) determined.

⇒ Inventorying will continue tomorrow, and a PCR reaction may be attempted. Protocols for the PCR purification kit (Miniprep) will be modified during this time, assuming the PCR reaction proceeds. No method currently exists to determine PCR outcome, as no gels or gel mix is available in the lab.