Team:Cornell/Week 1
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Results |
Protocol |
Notebook |
Parts Submitted
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June 5th - June 11th
Sunday
Monday
Tuesday
- CUgem bootcamp training session (day 1)
- Part I
- 1. PCR setup
- 2. Run an agarose gel and Gel purify the samples using Qaigen Kit.
- 3. Quantify the samples using nanodrop.
- 4. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
- 5. PCR clean up the samples and quantify using nanodrop.
- Part II
- 1. Miniprepping the overnight culture using Qaigen Kit.
- 2. Quantify the samples using nanodrop.
- 3. Digest 1μg of DNA sample for 2hours in the 37°C waterbath for 2hours.
- 4. Add CIAP to the backbone and put in the 50°C waterbath for 5minutes.
- 5. Run the samples on the agarose gel.
- 6. Cut out the band of right size, gel purify and quantify with nanodrop.
- Part I
Wednesday
- Weill Hall labspace introduction by Dr.Archer
- Cugem bootcamp training session (day 2)
Thursday
- Cugem bootcamp training session (day 3)
Friday
- Team meeting
- Organized the new lab space in Weill.
- Ordered the materials needed for the labwork.