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Team:Tsinghua
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Team
The team from Tsinghua University is comprised of undergraduates from the School of Life Sciences.
This year we want to exploit the value of E. Coli that is often overlooked, piggy-backing the metabolites as they come across.
'E Col Transporter'
Overview
This project is destined to generate several E. Coli strains which will cooperate with each other to move forth and back and transport any desired protein along a gradient. Module composition: binding, releasing, transition, movement
Parts
We built a host of parts during our project. The idea is that after every small step, we store our sequence as a biobrick part.
This strategy marks our progress and facilitates future use of these sequences.
Support
Our project is supported by School of Life Sciences, Department of Physics, Academy of Arts and Designs in Tsinghua.
Besides, we cited from a series of references.
The whole project is the work of Tsinghua iGEM Team comprising of 11 undergraduates. During the process, we shared the duty but every one has distinct focuses. The project was mainly designed by Yunxiao Zhang. Majority of the molecular cloning is done by Pan Deng, Guanqiao Li, Liyuan Zhu, and Xiao Shi. As for the protein purification and verification of our parts, Li Li, Yu Zhao and Chen Chen did most of the lab work. Our advisor Xiaozhi Fu kindly provided the guidance for using the equipment and chemicals in the lab while our director Dr. Guoqiang Chen helped us draft the flowchart of this project.
Human Practice
We put safety as our first priority and made a detailed safety brochure.
We also devoted efforts to publicize synthetic biology and to cooperate with other teams. The teams in China held a summer meetup to discuss our progress and share our resources. We also held a lecture introducing iGEM and our project in Tsinghua Univeristy. To get further support, we sought the cooperation of iGEM Team at Macquire, Australia.
