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Team:Cornell/Week 10
From 2011.igem.org
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August 7th - August 13th
Sunday
Morning lab work done by: Youjin Cho and Claire Paduano
- Objective
- Prepare VioE in Avi-Tagged pZE12 vector backbone for VioA and VioB gene inserts
- Digestion Setup
- 23.65μL H2O
- 18.6μL VioE in Avi-Tagged pZE12 vector backbone (1μg)
- 5μL 10x NEBuffer 2
- 0.5μL 100x BSA
- 1.25μL KpnI
- 1μL HindIII
- 50μL Total
- KpnI has only 75% efficiency in Buffer 2, so added 0.25uL more KpnI to digestion mixture
- Incubate in 37°C water bath for 2 hours
