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Team:DTU-Denmark-2/Notebook/Lab notes
From 2011.igem.org
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Lab notes
June
July
Tuesday 19.07.2011
First day in the lab and 48 biobricks to go, how exciting!
Of the 48 biobricks we will have when the project is finished we had only established 17 of them and ordered primers. We started the day with 11 PCR reactions of the first bash of 17 biobricks, we still need some DNA templates to due all 17. 6 PCR were correct and was purified with a purification Kit (GE Healthcare). So only 42 biobricks to go!
See protocol for PCR mix and program used.
- Insert GEL Photo
August
Thursday 04.08.2011
Today we purified 22 biobricks, which have been obtained over the last week of laboratory work with PCR. The purification of DNA was done by using the illustra GFX PCR DNA and Gel Band Purification Kit (GE Healthcare) according to manufactures manual for purification of DNA from TAE and TBE agarose gels protocol, see protocol section for more information.
- HeLa cells U2OS
- pyrG TEMP
September
October
