Sensor: Week 10 July 18-22

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Monday, July 18

The sensor group amplified the following constructs through the PCR protocol using the reverse primer:

B0030+C0051
B0031+C0051
B0034+C0051

Tuesday, July 19

Protocol Part Involved in Protocol
Restriction Digest Inserts using EcoRI and SpeI: B0030 + C0051 (R)
B0031 + C0051 (R)
B0034 + C0051 (R)
Or
B0034
Vectors using EcoRI and XbaI: Or34mcherry
34Cro
Tev
Ligation B0030 + Or34mcherry
B0031 + Or34mcherry
B0034 + Or34mcherry
Or + 34Cro
B0034 + Tev
Transformation/Plating The above ligations were transformed into
Escherichia coli cells and plated onto kanamycin resistant plates.



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