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From 2011.igem.org

Edward

Minipreps

• on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
• on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
• on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL

  
  

  Restriction Digest of RBS-spoVg and T7 RNA polymerase

  • BBa_K145001 cut by XbaI and PstI
  • BBa_K143021 cut by SpeI and PstI

    
    Final volume : 20 µL

    </p><p style="display:block;">DNA Gel electrophoresis (T7 RNA polymerase) </p><p style="display:block;">Protocol (6 wells) </p>

    • 1kb ladder
    • T7 undigested plasmid
    • Digested T7 (BBa_K145001 cut by XbaI and PstI)
    

    

    ladder - non digested - digested T7