Team:Paris Liliane Bettencourt/Notebook/2011/07/18/

From 2011.igem.org

Revision as of 13:34, 4 August 2011 by Kwarteng2 (Talk | contribs)

Edward

Minipreps

  • on BBa_I712074 (pT7), concentration of DNA=249.5ng/µL
  • on BBa_K145001 (T7 RNA polymerase), concentration of DNA=220.4ng/µL
  • on BBa_K143021 (RBS-spoVg colony 17), concentration of DNA=165.9ng/µL


    Restriction Digest of RBS-spoVg and T7 RNA polymerase

    • BBa_K145001 cut by XbaI and PstI
    • BBa_K143079 cut by SpeI and PstI </p><p style="display:block;">
      Final volume : 20 µL