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From 2011.igem.org

Wednesday

Lab work

• Ligation of A2,2 and B1 in PSB1C3 O/N 4 degrees.

• Run gel on the PCR products from yesterday: B1 (old) in expr. vector, B1 (new) in PSB1C3 again, A1 in PSB1C3. It seems that either the primers for the B1's arent working - or B1 is not insertet in the plasmids. There was a positive result for A1 - maybe we've got our first biobrick made!! Yayyyy:D

Other work

Checked out how the User Cloning system works. We will use this method on our human Cytochrome P450s. The method is now being optimized by the DTU-Denmark 2 team, and we are therefore getting help from them.

[http://www.neb.com/nebecomm/products/productE5500.asp NEB User Cloning Kit]

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