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From 2011.igem.org

PDMS 2 layer device fabrication 16.05.2011

Materials:

• flow and control layer molds • Sylgard 184 silicone elastomer kit: Base part and a Curing agent • TMCS

Method:

• Place molds into a TMCS vapor chamber • Control layer mixture: 20g Base + 4g Curing agent • Mix for 1 minute, degas for 2 minutes (standard protocol) • pour onto control layer mold and place mold in vacuum chamber • Flow layer mixture: 20g Base + 1g Curing agent • Mix for 1 minute and degas for 2 minutes (standard protocol) • Spin coat onto flow layer at 2200-2400rpm for 35secs (so far you can use my protocol on a spin coater) • Remove control layer mold from vacuum chamber, making sure no bubbles are left on the surface (remove with a toothpick if you see some ) • Place the control and flow layer in a 80C convection oven and incubate for 30 minutes (timing is critical here!) • Remove casts from oven, cut out control layer, punch holes, and align to flow layer • Put aligned device back into 80C oven and incubate for at least 90 minutes (and here you can increase the backing) • Remove devices from oven and punch flow layer holes