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Team:USTC-China/Notebook
From 2011.igem.org
2011-5-24
Genome Extraction of Top10
- Aim: to get the genome of Top10 for cheZ
- Time:2011-5-23
- Members: Duo An, Mengping Sun & Yinghong Lan
- Result:from the electrophoresis result, we can say we have basically got the whole genome of Top10.
- Notes: EP1:60ng/ul EP2:200ng/ul
2011.6.28 --- cultivate the bacteria of CheZ deficiency(RP1616),and the Control group(RP437). check the resistibility of RP1616 and RP437 result:both of the two groups are of none resistibility. members:Siwei Luo, Fangzhou Zhao, Zhilin Chen...
2011.6.29 --- check the motility of RP1616 strain and RP437 strain cultivate Top10 strain result: the bacterial plaque of RP1616 is smaller than that of RP437, implying that RP1616 is of CheZ deficiency, causing the decreased motility of the bacteria.
6.30
prepare for the competent cell of Top10 strain cultivated on 6.29 extract the genome of Top10 strain and use it as complates to run PCR of CheZ result: the concentration of PCR result is too low to continue the experiments.
7.1 --- conduct PCR of CheZ again ligate the CheZ DNA segment to plasmid PSb1C3, and transform it into Top10 competent cells result:如图
7.2 --- pick up single colony from the Petri dish, in which the competent cells are cultivated
