Team:Freiburg/Notebook/1 August

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Revision as of 14:30, 1 August 2011 by SophieCramer (Talk | contribs)


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME: Sophie PCR


Name: Sophie


Date: 01.08.11
Continue from Experiment: PCR (Date): 28.07.11

(Name): Sandra, Sophie

Project Name: Blue Light

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20 Name
10µl 5x Phusion Buffer of Primer
2.5µl Primer fw P35
2.5µl Primer dw P36
1µl dNTPs of Template DNA
1µl DNA-Template M35 (LovTAP)
0.5 µl Phusion (add in the end)

What program do you use?

LovTAP ohne Ueberhaenge


To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.


How did you label the PCR-Product, where is it stored and what do you do next?

Labelled: M35 short; stored in Gibson Stuff box.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

NAME OF YOUR EXPERIMENT

Investigators:NAME


Precipitator

PCR


Name: Sophie


Date: 01.08.11
Continue from Experiment: replay of PCR 19.07.11 (Date) 19.07.11

(Name) Ruediger

Project Name: GFP Pbd

PCR-Mixture for one Reaction:

For a 50 µl reaction use


32,5µl H20 Name
10µl 5x Phusion Buffer of Primer
2.5µl Primer fw P28
2.5µl Primer dw P18 / P19/ P20
1µl dNTPs of Template DNA
1µl DNA-Template S 14 (GFP)
0.5 µl Phusion (add in the end)

What program do you use?

Ruediger PCR (modified by Tobi and me. First 10 cycles with 55°C, next cycles with 62°C annealing temperature.

To confirm the PCR-Product has the correct size, load 2 µl of the sample onto an agarose-gel.


How did you label the PCR-Product, where is it stored and what do you do next?

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