Thursday, July 28

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LABLED 6 CENTERFUGE TUBES

T3 R3 P3

T4 R4 P4

Removed 6 mutagenesis tubes from freezer labled as Mutagenesis P,R and T products grown up and spun down

Lysis by Alkaly

The contents of these tubes was vortex-ed and poured into its corresponding centerfuge tube above each tube was rinsed with about 1 ml nuclease free water and poured into the corresponding centrifuge tubes.

These six centerfuge tubes were subsequently centerfuged at 4 degrees C. at 12,000 rpm for 30seconds twice. each tube was then decanted and wicked without disturbing the supernatant.

6 mLs of Solution I was added to each tube and vortex-ed to re-suspend the pellet.